Temporary

Part:BBa_K4013001

Designed by: Chang le   Group: iGEM21_Whittle   (2021-10-20)


P0452

P0452 is an endogenous promoter of cyanobacteria PCC6803 with an RBS, so CDS can be added directly through the restriction endonuclease digestion site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization:
These two native promoters are relatively weak in PCC 6803. We made this choice because excessive IAA would inhibit plant growth. For the terminator, we used a strong terminator called TrrnB.

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Categories
Parameters
None